Imaging Gene Expression in Live Cells and Tissues
نویسندگان
چکیده
منابع مشابه
Imaging gene expression in live cells and tissues.
Monitoring gene expression is crucial for studying the responses of gene therapy and clarifying gene function in various environments. Molecular imaging is a powerful tool for noninvasive visualization of gene expression. This article summarizes the current status of fluorescence and bioluminescence imaging (BLI) of gene expression in live cells and tissues, with the emphasis mainly on the earl...
متن کاملImaging of gene expression in living cells and tissues.
It is possible to observe gene expression within single cells using a tetracycline inducible promoter for activation. Transcription can be observed by using a fluorescent fusion protein to bind nascent RNA. Ultimately, it is desirable to activate a reporter gene within a single cell with only photons. This is achieved by preparing a chemically altered transcription factor that is functionally u...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2011
ISSN: 1559-6095
DOI: 10.1101/pdb.top103